Protocols Search for:SearchReset Search Showing: All results 5-FU injections B/T lymphopoiesis potential assay protocol Chipmentation for HSCs and HPCs CITE-seq. antibody staining protocol December 2022 Common stock solutions Freezing down cells Genotyping HSC-derived colonies by barcoded NGS gRNA/CRISPR-Cas9 editing of primary mouse hematopoietic cells Updated Summer 2024 HSC and HPC staining and sorting for RNA, Western blot, transplantation or ATAC_seq KI-67 staining of HSCs Lentiviral infection of HSCs for transplantation Lentivirus protocol Modified ATAC seq protocol 50K HSCs or HPCs Mouse iPSC general culture protocol Nuclear extraction and co-immunoprecipitation protocol Nucleofection of mouse iPSCs, colony picking, and genotyping Reagent databases Sick mouse take down Site-directed mutagenesis in very large cDNA Soluplus HSC cultures Western blot protocol